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. 2014 Nov 5;105(11):1472–1479. doi: 10.1111/cas.12538

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© 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Fig 1 — Expression levels of CD203c-positive basophils after in vitro exposure to carboplatin (CBDCA) and wortmannin, a phosphatidylinositol 3-kinase inhibitor (measured by flow cytometric analysis). Whole blood with or without wortmannin was stained for CD3, prostaglandin D2 receptor (CRTH2), and CD203c. Flow cytometer charts for CD3− and CRTH2 + cells (basophils) are shown. Upregulation of CD203c on basophils (shown as a percentage in (a)) was determined using a threshold that was defined as the expression level above which 2% of basophils in the negative control column fluoresce, on average. (a) Data are from the patient whose response to CBDCA was highest among the hypersensitivity reaction-positive patients. This patient's basophils were pretreated with the indicated concentrations of wortmannin, and subsequently exposed to the negative control, positive control, and 50 μg/mL CBDCA. Percentages shown indicate the upregulation rate of CD203c. Mean fluorescence intensities (MFIs) indicated for binding levels of CD203c on basophils. (b) Difference between the respective mean upregulation rates (n = 2) of three patients were analyzed using one-way repeated measures anova.