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. 2014 Nov 5;105(11):1472–1479. doi: 10.1111/cas.12538

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© 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

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Fig 2 — Binding levels of CRA1, CRA2, and anti-IgE antibody measured by basophil staining to confirm the passive sensitization by flow cytometric analysis. (a) Immunostained healthy subject's basophils with anti-FcεRI antibodies; PBMCs with or without acid treatment were stained for CD3, prostaglandin D2 receptor (CRTH2), and FcεRI. (b) Immunostained healthy subject's basophils with anti-IgE antibody; PBMCs with or without acid treatment were stained for CD3, CRTH2, and IgE. Flow cytometer charts for CD3⁻ PBMCs are shown. Basophil fraction (CD3⁻ and CRTH2⁺ PBMC) was gated. MFIs indicated for binding levels of anti-CRA1, anti-CRA2, and anti-IgE antibodies on basophils. MFI, mean fluorescence intensity.