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. 2004 Jul;42(7):3164–3168. doi: 10.1128/JCM.42.7.3164-3168.2004

FIG. 2.

FIG. 2.

Determination of the analytical sensitivity of previously published nested PCR assays for B. burgdorferi (11) and A. phagocytophilum (17). The results from the amplification of 10-fold serial dilutions ranging from 125 to 1.25 × 10−3 A. phagocytophilum-infected HL-60 cells (upper lanes 1 to 6) and from 5 × 105 to 5 B. burgdorferi spirochetes (lower lanes 1 to 6) are shown on a 2% agarose gel stained with ethidium bromide. Lanes 7 are negative controls, and lanes M contain phage φX174 DNA digested with HaeIII.