TABLE 1.
Effect of DNA background on the amplification of B. burgdorferi
| Dilutiona | Threshold cycles (Ct) in various DNA backgroundsb
|
|||
|---|---|---|---|---|
| Water | Human | Tick | Mouse | |
| 10−1 | 20.6, 20.7, 20.9 | 20.6, 20.3, 20.2 | 20.7, 20.6, 20.4 | 20.1, 20.4, 20.5 |
| 10−2 | 23.9, 23.7, 23.8 | 23.6, 23.8, 23.7 | 23.9, 23.7, 23.8 | 23.6, 23.7, 23.7 |
| 10−3 | 27.5, 27.7, 27.6 | 27.8, 27.2, 27.6 | 27.5, 27.2, 27.8 | 27.0, 27.3, 27.2 |
| 10−4 | 31.1, 31.1, 31.7 | 31.3, 31.8, 30.9 | 30.4, 30.6, 30.6 | 30.7, 31.4, 30.9 |
| 10−5 | 35.9, 36.3, 35.2 | 34.7, 34.6, 35.5 | 34.7, 34.4, 34.5 | 34.1, 34.0, 33.5 |
a
Values represent the dilution factors using DNA extracted from B. burgdorferi strain B31 grown in BSK-H medium. The diluent for each dilution series consisted of undiluted DNA extracted from 200 μl of EDTA-treated whole blood (human), a single adult I. scapularis tick (tick), or 50 μl of EDTA-treated whole blood (mouse).
b
Each value represents the threshold cycle from one of three separate real-time multiplex PCR amplifications.