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Binding of polyclonal phage-displayed antibodies to intimin (A) and EspA120 (B). Three rounds of panning were carried out against MBP-intimin188 plus MBP-intimin282 or MBP-EspA120. MBP-intimin188 plus MBP-intimin282 (dark gray bars, panel A), MBP-EspA120 (dark gray bars, panel B) or MBP (light gray bars) were immobilized on ELISA plates (500 ng/well) and detected with 1012 CFU of phage/ml (100 μl/well) from panning rounds 0, 1, 2, and 3 (R0 to R3). Absorbances were measured at 405 nm.
