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Detection of intimin (A) and EspA (B) with monoclonal phage-displayed antibodies. MBP-intimin282 (dark gray bars, panel A), MBP-intimin188 (open bars, panel A), MBP (light gray bars), and EspA120 (dark gray bars, panel B) were immobilized (1 μg/well) and detected with 5 × 1012 CFU of monoclonal phage/ml (100 μl/well) isolated by panning against intimin (panel A, scFv I1 to I6) or EspA120 (panel B, scFv E1 to E3). Absorbances were measured at 405 nm.
