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. 2004 Jul;42(7):2966–2976. doi: 10.1128/JCM.42.7.2966-2976.2004

FIG. 9.

FIG. 9.

One-step detection if intimin with AP-scFv fusion proteins. I3-AP fusion (panel A, 5 ml of culture supernatant/blot) was used to detect 500 ng of MBP-intimin282 (lane 1), MBP-intimin188 (lane 2), and MBP (lane 3)/lane in Western blots. Antibody-AP fusions (100 μl of culture supernatant/well) were also used to detect intimin282 (dark gray bars), intimin188 (open bars), and MBP (light gray bars) by ELISA (1 μg/well, panel B). For both assays, binding was detected by the addition of appropriate AP substrate.