Table 1. Primer sequences used for RT-PCR.
| Gene description | Sequences (5′→3′) | Annealing temperature (°C) | PCR product (bp) |
|---|---|---|---|
| Wingless-type MMTV integration site family, member 10b (Wnt10b) | F: TTTTGGCCACTCCTCTTCCT | 61 | 183 |
| R: TCCTTTTCCAACCGAAAACC | |||
| Dickkopf 1 (DKK1) | F: GGTGCACACCTGACCTTCTT | 59 | 144 |
| R: GAGGGGAAATTGAGGAAAGC | |||
| Low-density lipoprotein receptor-related protein 5 (LRP5) | F: AAGGGTCCACAAGGTCAAGG | 55 | 520 |
| R: AGAAGCACAGATGGCTGCAC | |||
| Frizzled receptor 1 (FZDR1) | F: ATGAACAGGCCTTTCGTTCT | 55 | 484 |
| R: CCTCGTGTAGAACTTCCTCC | |||
| Lymphoid-enhancer-binding factor (LEF1) | F: CCAGCTATTGTAACACCTCA | 55 | 420 |
| R: TTCAGATGTAGGCAGCTGTC | |||
| Cyclin D1 (Cyc-D1) | F: TGGGAAGTTTTGTTGGGTCA | 55 | 144 |
| R: TCCTTGTCCAGGTAATGCCA | |||
| Insulin-like growth factor (IGF-1) | F: TCAACAAGCCCACAGGGTAT | 60 | 280 |
| R: ACTCGTGCAGAGCAAAGGAT | |||
| Hepatocyte growth factor (HGF) | F: CGAGGCCATGGTGCTATACT | 54 | 290 |
| R: ACACCAGGGTGATTGAGACC | |||
| Vascular endothelial growth factor (VEGF) | F: TCTTCAAGCCATCCTGTGTG | 60 | 165 |
| R: GCGAGTCTGTGTTTTTGCAG | |||
| Keratocyte growth factor (KGF) | F: GACATGGATCCTGCCAACTT | 54 | 686 |
| R: AATTCCAACTGCCACTGTCC | |||
| Glyceraldehyde-3-phosphatedehydrogenase (GAPDH) | F: AGAACATCATCCCTGCATCC | 60 | 321 |
| R: TCCACCACCCTGTTGCTGTA |