Skip to main content
. 2015 Jun 10;10(6):e0130194. doi: 10.1371/journal.pone.0130194

Fig 3. miR-135b directly regulates TGFBR2 expression at the post-transcriptional level.

Fig 3

(A) Quantitative RT-PCR analysis of the miR-135b levels in HT-29 and SW-480 cells treated with scrambled pre-miR-control, pre-miR-135b, anti-miR-control or anti-miR-135b. (B and C) Western blot analysis of the TGFBR2 protein levels in HT-29 and SW-480 cells treated with pre-miR-control, pre-miR-135b, anti-miR-control or anti-miR-135b. B: representative image; C: quantitative analysis. (D) Quantitative RT-PCR analysis of the TGFBR2 mRNA levels in HT-29 and SW-480 cells treated with pre-miR-control, pre-miR-135b, anti-miR-control or anti-miR-135b. (E) Direct binding of the TGFBR2 3’-UTR to miR-135b. HT-29 and SW-480 cells were co-transfected with a firefly luciferase reporter containing either wild-type (WT) or mutant (Mut) miR-135b binding sites in the TGFBR2 3’-UTR and either pre-miR-control or pre-miR-135b. Twenty-four hours after transfection, the cells were assessed using a luciferase assay kit. The results are displayed as the ratio of firefly luciferase activity in the miR-135b-transfected cells to that in the control cells. * P < 0.05; ** P < 0.01; *** P < 0.001.