(A and B) Western blot analysis of the TGFBR2 protein levels in HT-29 cells treated with scrambled control siRNA, TGFBR2 siRNA, control vector or TGFBR2 vector. A: representative image; B: quantitative analysis. (C) CCK8 cell viability assays were performed 12, 24, 36, 48 and 60 h after the transfection of HT-29 cells with either scrambled control siRNA or TGFBR2 siRNA. (D) CCK8 cell viability assays were performed 12, 24, 36, 48 and 60 h after the transfection of HT-29 cells with either control vector or TGFBR2 vector. (E) Apoptosis assays were performed after the transfection of HT-29 cells with scrambled control siRNA, TGFBR2 siRNA, control vector or TGFBR2 vector. Left panel: representative image; right panel: quantitative analysis. * P < 0.05; ** P < 0.01.