FIG 7 .

Ultrastructure of persistent LGTV TP21 infection in 293T cells. Acutely infected cells (A and D) were infected at an MOI of 10 and fixed at 72 hpi. Persistently infected cells were obtained following infection at an MOI of 0.1 and passaged upon reaching confluence. Panels B and E represent cells passaged 10 times, and panels C and F represent cells passaged 31 times. After fixation, cells were embedded in resin and 70-nm sections were cut and processed for TEM. Lower-magnification panels A to C show ER expansion (ER), vesicles (Ve), tubules (T), and virions (Vi). Higher-magnification images are shown in panels D to F. Panel F depicts convoluted membrane accumulations that were common throughout all of the samples.