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. 2015 Jun 9;6(3):e00392-15. doi: 10.1128/mBio.00392-15

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Copyright © 2015 Chen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 8 — The ywbHG operon is directly induced by acetate as well as by glycerol. (A) A cartoon model showing induction of the ywbHG operon by acetate. YwbI is a LysR-type transcription factor predicted to regulate ywbHG in response to acetate. (B) Assays of the β-galactosidase activities of the wild-type cells (white bars, YC259) or the ΔywbI mutant cells (gray bars, YC1245) harboring the P_ywbHG-lacZ transcriptional reporter in shaking LB in the absence or presence of 0.2% NaAc or 0.2% KAc. (C) ywbHG expression was induced by glycerol. B. subtilis cells harboring the P_ywbHG-lacZ transcriptional fusion (YC259) were streaked out on LB agar plates plus X-Gal and without or with supplementation of 1% glycerol.