TABLE 2.

Sequences of type-specific nested PCR primers used in this study

Primer cocktail	HPV genotype	Amplicon (bp)	Sequence (5′-3′)	Position (bp)
I	16	457	CAC AGT TAT GCA CAG AGC TGC	141-161
			CAT ATA TTC ATG CAA TGT AGG TGT A	597-573
	18	322	CAC TTC ACT GCA AGA CAT AGA	170-190
			GTT GTG AAA TCG TCG TTT TTC A	491-470
	31	263	GAA ATT GCA TGA ACT AAG CTC G	137-158
			CAC ATA TAC CTT TGT TTG TCA A	399-378
	59	215	CAA AGG GGA ACT GCA AGA AAG	159-179
			TAT AAC AGC GTA TCA GCA GC	373-354
	45	151	GTG GAA AAG TGC ATT ACA GG	82-101
			ACC TCT GTG CGT TCC AAT GT	232-213
II	33	398	ACT ATA CAC AAC ATT GAA CTA	172-192
			GTT TTT ACA CGT CAC AGT GCA	569-549
	6/11	334	TGC AAG AAT GCA CTG ACC AC	201-220
			TGC ATG TTG TCC AGC AGT GT	534-515
	58	274	GTA AAG TGT GCT TAC GAT TGC	297-317
			GTT GTT ACA GGT TAC ACT TGT	570-550
	52	229	TAA GGC TGC AGT GTG TGC AG	178-197
			CTA ATA GTT ATT TCA CTT AAT GGT	406-383
	56	181	GTG TGC AGA GTA TGT TTA TTG	294-314
			TTT CTG TCA CAA TGC AAT TGC	475-455
III	35	358	CAA CGA GGT AGA AGA AAG CAT C	157-178
			CCG ACC TGT CCA CCG TCC ACC G	514-493
	42	277	CCC AAA GTA GTG GTC CCA GTT A	85-106
			GAT CTT TCG TAG TGT CGC AGT G	361-340
	43	219	GCA TAA TGT CTG CAC GTA GCT G	102-123
			CAT GAA ACT GTA GAC AGG CCA AG	320-298
	44	163	TAA ACA GTT ATA TGT AGT GTA CCG	248-271
			TAT CAG CAC GTC CAG AAT TGA C	410-389
IV	68	333	GCA GAA GGC AAC TAC AAC GG	4049-4068
			GTT TAC TGG TCC AGC AGT GG	4381-4362
	39	280	GAC GAC CAC TAC AGC AAA CC	213-232
			TTA TGA AAT CTT CGT TTG CT	492-473
	51	223	GAG TAT AGA CGT TAT AGC AGG	319-339
			TTT CGT TAC GTT GTC GTG TAC G	541-520
	66	172	TTC AGT GTA TGG GGC AAC AT	353-372
			AAA CAT GAC CCG GTC CAT GC	520-501