TABLE 2.
PCR primers used in this study
| Primer name and HPV type | Sequence (5′-3′)a | Position |
|---|---|---|
| MY09 | CGT CCM ARR GGA WAC TGA TC | 7033-7014 in HPV-16 |
| MY11 | GCM CAG GGW CAT AAY AAT GG | 6582-6601 in HPV-16 |
| GP6+ | GAA AAA TAA ACT GTA AAT CA | 6765-6746 in HPV-16 |
| HMB01b | GCG ACC CAA TGC AAA TTG GT | |
| GAPDH-LEFT | TCA ACG GAT TTG GTC GTA TT | 77-96 |
| GAPDH-RIGHT | TAG AGG CAG GGA TGA TGT TC | 664-645 |
| pSP6 | ATT TAG GTG ACA CTA TAG AA | 139-158 in pGEM T Easy |
| pT7 | TAA TAC GAC TCA CTA TAG GG | 2999-3 in pGEM T Easy |
| TOPO-LEFT | AGC TTG GTA CCG AGC TCG GAT | 255-235 in pCR2.1-TOPO |
| TOPO-RIGHT | CGA ATT GGG CCC TCT AGA TGC | 363-343 in pCR2.1-TOPO |
| MY09GAP | CGT CCM ARR GGA WAC TGA TC TCA ACG GAT TTG GTC GTA TT | |
| MY11GAP | GCM CAG GGW CAT AAY AAT GG TAG AGG CAG GGA TGA TGT TC |
a
Nucleotide abbreviations are according to the International Union of Pure and Applied Chemistry system of nomenclature: M, A + C; R, A + G; W, A + T; Y, C + T.
b
HMB01 is directed to the minus strand of HPV-51.