Fig. 2.

Quantification of CVB4 RNA and level of infectious particles. Fifty microliters of the culture supernatant fluid containing CVB4 was applied to petri dish lids in quadruplicate. Inocula on lids were dried for 2 h at room temperature, and, thereafter, recovered with 1 mL of culture media. The infectious titers were determined and expressed as log₁₀ TCID₅₀ 50 μL⁻¹ (■). RNA was extracted and the levels of viral RNA were measured by quantitative RT-PCR and expressed as C_t (□). The results are the mean + SD of four independent experiments.