Fig. 3.

Resuscitation of Tomitella biformata in the presence of recombinant Rpf expressed in Rhodococcus erythropolis M1218. (A) Non-dividing T. biformata cells (37.2 cells mL⁻¹) were inoculated into mMMF liquid medium containing final rRpf protein concentrations of 10 nM (○), 1 nM (Δ), 0.1 nM (♦), and 0 nM (⋄) control. (B) T. biformata (1.67×10⁵ CFU mL⁻², 99.9% non-dividing cells) inoculated onto TSBF agar medium containing final rRpf protein concentrations of 300 pM (●), 30 pM (▲), 3 pM (■), and 0 pM (⋄) control. Non-dividing T. biformata cells were obtained after a 60-day incubation under oxygen limited conditions. Cells were washed and diluted prior to inoculation into growth media. (n=3 per Rpf treatment, error bars represent SE)