Table 1.

Number of sequences determined from plates exposed to increasing doses of Tomitella biformata recombinant Rpf^a

Rpf concentration (pM)	Number of colonies sequenced	Number of sequences obtaineda	Number of non-ambiguous sequencesb
0	8	8	8
1.25	23	21	6
12.50	28	28	15
125.00	25	25	17
1250.00	108	95	78
Total	192	177	124

^aTomitella biformata Rpf cloned into the pTip vector (20) and expressed in Rhodococcus erythropolis M1218.

^bSequences may not have been produced if cells were insufficiently lysed

^cAfter removing sequences with any uncalled bases that could not be resolved from the sequence trace files.