Table 3.
Major taxonomic groups found by 16S rRNA gene amplicon sequencing (%)
| phylum | order | genus | control | HRC | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
|
|||||||||||
| day 13 | day 40 | day 73 | day 88 | day 115 | day 13 | day 40 | day 73 | day 88 | day 115 | |||
| Actinobacteria | Actinomycetales | Propionicimonas | <0.1 | <0.1 | ND | ND | ND | ND | 2.8 | 4.2 | 5.1 | 2.0 |
| Bacteroidetes | Bacteroidales | Bacteroides | ND | <0.1 | <0.1 | <0.1 | <0.1 | ND | ND | <0.1 | ND | <0.1 |
| Blvii28 | 0.1 | <0.1 | <0.1 | <0.1 | 0.1 | 0.2 | <0.1 | 0.1 | 0.1 | ND | ||
| unassigned 1 | 1.1 | 0.4 | 1.7 | 1.6 | 0.9 | 3.0 | 0.2 | 1.1 | 1.8 | 1.3 | ||
| unassigned 2 | 0.2 | <0.1 | 0.1 | <0.1 | <0.1 | 0.3 | <0.1 | ND | ND | 0.1 | ||
| Chloroflexi | Dehalococcoidales | Dehalococcoides | <0.1 | ND | ND | <0.1 | ND | ND | ND | ND | ND | ND |
| Firmicutes | Bacilli | unassigned 3 | <0.1 | <0.1 | <0.1 | ND | ND | 0.1 | <0.1 | ND | ND | ND |
| Clostridiales | Anaeromusa | ND | ND | ND | ND | ND | 2.2 | 8.9 | 6.3 | 4.0 | 0.6 | |
| Pelosinus | <0.1 | ND | ND | ND | ND | 24.4 | 0.6 | 0.3 | 0.5 | 0.2 | ||
| unassigned 4 | <0.1 | ND | <0.1 | ND | ND | <0.1 | 0.1 | <0.1 | 0.6 | 0.2 | ||
| unassigned 5 | <0.1 | ND | ND | <0.1 | <0.1 | 12.9 | 0.2 | 0.7 | 1.1 | 0.3 | ||
| Proteobacteria | Sphingomonadales | Sphingomonas | 5.8 | 10.1 | 13.5 | 16.0 | 11.1 | 0.7 | 10.0 | 10.5 | 10.3 | 13.9 |
| Burkholderiales | Janthinobacterium | 28.5 | 58.1 | 59.3 | 55.9 | 50.7 | 2.9 | 44.7 | 52.6 | 49.6 | 55.2 | |
| Desulfovibrionales | Desulfovibrio | <0.1 | <0.1 | <0.1 | <0.1 | 0.1 | 4.8 | 0.7 | 2.5 | 3.4 | 1.6 | |
| Desulfuromonadales | Geobacter | 1.7 | 0.3 | 0.8 | 1.2 | 2.6 | 6.6 | 0.5 | 2.5 | 5.2 | 1.6 | |
| Pseudomonadales | Pseudomonas | 7.3 | 26.9 | 13.8 | 12.3 | 10.8 | 4.6 | 24.6 | 13.0 | 10.6 | 15.5 | |
| Spirochaetes | Spirochaetales | Treponema | 0.2 | 0.1 | 0.2 | 0.2 | 0.4 | 0.5 | 0.5 | 0.8 | 0.7 | 0.7 |
|
| ||||||||||||
| phylum | order | genus | TM-B | EDC | ||||||||
|
|
|
|||||||||||
| day 13 | day 40 | day 73 | day 88 | day 115 | day 13 | day 40 | day 73 | day 88 | day 115 | |||
|
| ||||||||||||
| Actinobacteria | Actinomycetales | Propionicimonas | ND | <0.1 | <0.1 | <0.1 | ND | 0.1 | 0.5 | 0.1 | <0.1 | <0.1 |
| Bacteroidetes | Bacteroidales | Bacteroides | 1.5 | 4.3 | 4.8 | 5.0 | 5.5 | 1.7 | 2.9 | 1.6 | 0.6 | 3.9 |
| Blvii28 | 2.4 | 1.1 | 3.0 | 2.0 | 4.7 | 5.6 | 3.6 | 5.9 | 2.3 | 20.0 | ||
| unassigned 1 | 1.4 | 26.1 | 16.7 | 11.5 | 27.9 | 7.6 | 11.6 | 19.9 | 16.4 | 17.7 | ||
| unassigned 2 | 62.0 | 29.1 | 31.3 | 29.1 | 20.5 | 45.9 | 31.4 | 23.6 | 18.9 | 8.1 | ||
| Chloroflexi | Dehalococcoidales | Dehalococcoides | ND | 0.5 | 1.8 | 1.7 | 0.9 | ND | <0.1 | 0.9 | 1.3 | 0.2 |
| Firmicutes | Bacilli | unassigned 3 | 0.2 | 1.8 | 0.6 | 0.5 | 1.6 | 9.2 | 15.4 | 9.2 | 3.8 | 3.4 |
| Clostridiales | Anaeromusa | ND | ND | <0.1 | <0.1 | <0.1 | ND | ND | ND | ND | ND | |
| Pelosinus | <0.1 | 0.1 | <0.1 | <0.1 | <0.1 | 0.2 | 0.1 | ND | 0.1 | <0.1 | ||
| unassigned 4 | 0.1 | 1.2 | 1.3 | 1.5 | 1.2 | 0.3 | 0.9 | 1.6 | 1.4 | 0.7 | ||
| unassigned 5 | 0.1 | 0.3 | 0.7 | 0.6 | 0.5 | 0.3 | 0.2 | 0.4 | 0.2 | 0.5 | ||
| Proteobacteria | Sphingomonadales | Sphingomonas | 0.1 | 1.5 | 1.7 | 1.7 | 1.5 | 0.7 | 1.3 | 2.5 | 6.1 | 3.6 |
| Burkholderiales | Janthinobacterium | 0.9 | 7.1 | 8.9 | 6.5 | 5.4 | 2.1 | 7.5 | 8.9 | 17.4 | 18.7 | |
| Desulfovibrionales | Desulfovibrio | 0.7 | 0.8 | 2.6 | 2.2 | 1.7 | 0.7 | 0.4 | 1.1 | 0.9 | 0.8 | |
| Desulfuromonadales | Geobacter | 0.4 | 0.9 | 1.1 | 1.2 | 0.4 | 0.5 | 0.8 | 1.9 | 2.4 | 1.1 | |
| Pseudomonadales | Pseudomonas | 2.4 | 3.4 | 2.4 | 1.7 | 1.6 | 0.7 | 3.9 | 2.4 | 4.5 | 4.0 | |
| Spirochaetes | Spirochaetales | Treponema | 0.6 | 2.8 | 6.4 | 9.2 | 5.9 | 0.4 | 0.8 | 2.7 | 6.5 | 2.3 |
ND, not detected