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. 2015 Jun 15;26(12):2181–2189. doi: 10.1091/mbc.E15-01-0014

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© 2015 Williams et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Overexpressing Grp170 or Sil1 markedly stimulates toxin release from BiP. (A) WCEs derived from 293T cells transfected with the indicated construct and intoxicated with or without CT were analyzed by SDS–PAGE and immunoblotted with the indicated antibodies. (B) WCEs derived from cells in A were subjected to immunoprecipitation using an antibody against CTA. The immunoprecipitates were analyzed by reducing SDS–PAGE and immunoblotted against the indicated antibodies. (C) As in B, except that G41L Grp170-FLAG or FLAG-H214A Sil1 was transfected instead of the respective WT counterpart.