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. 2015 Jun 15;26(12):2205–2216. doi: 10.1091/mbc.E14-09-1400

FIGURE 4:

FIGURE 4:

An extra copy of CLB5 restores near wild-type Clb5 expression in ubp15∆ cells. (A) Expression of Clb5 in asynchronous cultures of ubp15∆ cells carrying 1xCLB5-TAP (lane 1), 2xCLB5-TAP (lane 2), and 3xCLB5-TAP (lane 3). Cell extracts were immunoblotted with PAP antibodies to detect the TAP-tagged Clb5 proteins. (B) Wild-type and ubp15∆ cells carrying one or two copies of CLB5-TAP were released from G1 arrest and probed for the presence of Clb5 at the indicated times.