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. 2015 Jun 15;26(12):2217–2226. doi: 10.1091/mbc.E14-11-1563

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© 2015 Mackay and Ullman. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Replication stress leads to prolonged time to abscission. (A) Timeline of experimental procedure and montages of HeLa cells stably expressing GFP-tubulin (green) and histone H2B-mCherry (magenta) cultured in HU or APH. Scale bar, 20 μm. (B) Quantification of abscission timing using the assay described in Figure 2. ***p < 0.0001. (C) Cumulative frequency plots of progression through abscission. Each point represents the percentage of all midbody cells analyzed in B that had proceeded through abscission over the time course of the experiment. Error bars are mean and SD from three to five experiments.