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. 2015 Jun 15;26(12):2227–2241. doi: 10.1091/mbc.E15-01-0026

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© 2015 Karg, Warecki, and Sullivan. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/bync-sa/3.0).

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FIGURE 4: — Aurora B is highly associated with acentric fragments and their DNA tethers. (A) Still frames of a control neuroblast (no I-CreI expression) movie show GFP-tagged Aurora B (cyan) on kinetochores during metaphase and at the cleavage furrow during anaphase. Dotted lines outline the plasma membrane of the cell. Scale bars, 2 μm. (B) Still frames from a movie of a dividing neuroblast during the same mitotic stages with I-CreI–induced acentrics decorated with GFP-tagged Aurora B (cyan arrows). Undamaged chromosomes (cyan arrowheads) are not associated with Aurora B (bottom). These results are in accord with Royou et al. (2010). Dotted lines outline the plasma membrane of the cell. Scale bars, 2 μm.