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. 2015 Jun 15;26(12):2227–2241. doi: 10.1091/mbc.E15-01-0026

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© 2015 Karg, Warecki, and Sullivan. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/bync-sa/3.0).

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FIGURE 5: — Aurora B RNAi does not lead to gross changes in chromosome behavior or structure. (A) Measured size of undamaged chromosomes in I-CreI–expressing control (cyan triangles) and I-CreI; Aurora B RNAi neuroblasts (red squares) at the moment of maximal metaphase compaction. (B) Measured size of acentrics generated in I-CreI neuroblasts (cyan triangles) and I-CreI; Aurora B RNAi neuroblasts (red squares) at the beginning of NEF. (C) Distance between acentrics and the main chromosome mass in gap-forming, I-CreI–alone (control) neuroblasts (cyan triangles) and non–gap-forming I-CreI; Aurora B RNAi neuroblasts (red squares) after completion of anaphase. (D) Timing of the initiation of acentric poleward segregation after onset of anaphase in both I-CreI–alone (control) and I-CreI; Aurora B RNAi neuroblasts. The timing of the initiation of acentric poleward segregation was grouped into two categories: 0–4 min (cyan bars) and 4–7 min (red bars). (E) Still images from time-lapse movies of equal (top) and unequal (bottom) segregation of acentrics during anaphase in neuroblasts expressing I-CreI alone (top) or I-CreI plus Aurora B RNAi (bottom). Red arrows show acentrics. Scale bar, 2 μm. Right, graph of the percentage of unequal segregation in both types of neuroblasts. A statistically nonsignificant increase in unequal segregation of acentrics in I-CreI with Aurora B RNAi neuroblasts vs. I-CreI alone (control) neuroblasts was observed. (F) Single-frame pictures of third-instar neuroblasts from wild-type larvae (top) and larvae in which Aurora B is reduced (bottom). Right, graph of the percentage of binucleate neuroblasts in both types of larvae. Cyan arrows point to binucleate cells. Red channel is H2Av-RFP. Gray channel is differential interface contrast (DIC). Scale bar, 6 μm. p values were calculated by independent t test with equal variances not assumed.