Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 15;26(12):2242–2251. doi: 10.1091/mbc.E15-01-0041

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Zhang and Wang. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 1: — The deubiquitinase activity of VCIP135 is inhibited during mitosis. (A) Asynchronous HeLa cells were immunoprecipitated using control (Ctr) or VCIP135 (135) antibodies. All eight individual di-ubiquitin molecules were treated with the precipitates; this was followed by Western blot for ubiquitin. Lane numbers are denoted underneath the gels in this and all following figures. (B) Quantitation of A to show the ratio of Ub1 to the sum of Ub1 and Ub2. Results are expressed as the mean ± SEM from three independent experiments. (C) VCIP135 immunoprecipitated from asynchronous interphase (Int) or nocodazole-arrested mitotic (Mit) HeLa cells was incubated with K48- or K63-linked di-ubiquitin. Note that mitotic VCIP135 has reduced activity for both di-ubiquitin molecules.