FIGURE 5:
MCT1 and HMGCS2 are down-regulated in K8−/− under normal and ketogenic conditions. Whole lysates of colon from K8+/+ and K8−/− mice were analyzed by immunoblotting for MCT1. Equal loading is shown by Hsc70 (A). The immunoblots were quantified, and MCT1 was normalized against Hsc70 (B). The results represent the average ± SD, with significant differences shown as **p < 0.01. Two- to 2.5-mo-old K8+/+ and K8−/− male mice were subjected to normal conditions or 18 h of starvation, and lysates of isolated colonic epithelium were prepared. The samples were analyzed by immunoblotting for HMGCS2 and MCT1 (C). Equal loading is shown by Hsc70, and the absence of keratin filaments is shown by K8.