FIGURE 10:

The promoter of RTC3, a putative target of Hot1, is activated in hot1∆ cells. It is less efficiently activated in msn2∆msn4∆ cells and possesses several STREs. (A) The –803-RTC3-LacZ reporter gene was assayed in the indicated strains. (B) Top, the sequence of the RTC3 promoter. STREs are boxed, and the proposed Hot1-binding sites (Cook and O'Shea, 2012) are underlined. The most 5′ nucleotide of each deletion construct is marked by its number. Bottom, β-galactosidase activity of the indicated deletion constructs of the RTC3 promoter as assayed in wild-type cells.