FIGURE 12:

STL1 is the only gene whose expression is abolished in hot1∆ cells. mRNA levels of the indicated genes were assayed by real-time quantitative RT-PCR performed on RNAs isolated from hot1∆ cells harboring either an empty vector or a single copy of a genomic fragment carrying the HOT1 gene (including its native promoter). Cells were grown to mid log phase and then exposed to the indicated treatments. The value of each gene was normalized to the value of ACT1, which served as an internal control, and is shown as the ratio between its value in the sample to its value in untreated hot1∆/HOT1 cells. Values shown are averages of three independent experiments.