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. 2015 Jun 15;26(12):2357–2374. doi: 10.1091/mbc.E14-12-1626

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© 2015 Bai et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Short fragments of the STL1 promoter, cloned upstream of the CYC1 minimal promoter, are sufficient to render it responsive to osmotic pressure. Fragments derived from the upstream region of the STL1 promoter were fused to the minimal elements of the CYC1 promoter. Left, resulting constructs; right, β-galactosidase activities of cells harboring these constructs. All fragments in A share the same downstream endpoint (−533). The endpoint is −564 in most of the fragments tested in B except for the two bottom constructs, in which the endpoint is −583.