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. 2015 Jun 15;26(12):2357–2374. doi: 10.1091/mbc.E14-12-1626

FIGURE 8:

FIGURE 8:

The STL1 promoter and the HoRE are active in smp1∆ and ras2∆msn2∆msn4∆ cells but not in hog1∆ and hot1∆ cells. (A) The –704STL1-LacZ reporter gene was introduced into the indicated strains. Its activity was assayed in cells grown under optimal conditions and in response to 0.7 M NaCl. (B) Activity of the STL1(E1)CYC1-LacZ reporter was assayed in the indicated strains. (C) Overexpression of Hog1WT or Hog1D170A+F318L fails to activate the STL1 promoter in hot1∆ cells. (D) Overexpression of Hot1 fails to activate the STL1 promoter in hog1∆ cells.