TABLE 6:
Oligonucleotides used in this study.
| Primers for cloning and plasmid construction | |
| STL1-Rev | gactGGATCCggtcatggtctaaaactttctatg |
| STL1-704 | gactGTCGACataacggacgtacggac |
| STL1-665 | gactGTCGACtattccaccgcatttgg |
| STL1-654 | gactGTCGACatttggcccatttggctc |
| STL1-645 | gactGTCGACcatttggctcactttgac |
| STL1-636 | gactGTCGACactttgactcaacttgc |
| STL1-626 | gactGTCGACaacttgcgtcattttaac |
| STL1-583 | gactGTCGACtttttcggccaccgcata |
| STL1-550 | gactGTCGACtccgctacctgcatttg |
| STL1-400 | gactGTCGACtgtttctcggctatatac |
| STL1-704F | gactCTCGAGataacggacgtacggacg |
| STL1-533R | gactCTCGAGcaaatgcaggtagcggag |
| STL1-564R | gactCTCGAGtatgcggtggccgaaaaag |
| STL1-583R | gactCTCGAGacaaagtcggacccttc |
| CYC1-R | gactGGATCCGGTCATTATTAA gactCTCGAGcatttggcccatttggctcactttgactcaacttgcgtcattttaactgatatgaagggtcc |
| STL1-E1 | AGATCCGCCAGGCGTGTA gactCTCGAGcatttggcccatttggctcactttgactcaacttgcgtcattttaactgatatg |
| STL1-E2 | CAGATCCGCCAGGCGTGTA gactCTCGAGctattccaccgcatttggcccatttggctcactttgactcaacttgcgtcattttaactgatatg |
| STL1-E3 | CAGATCCGCCAGGCGTGTA gactCTCGAGctattccaccgcatttggcccatttggctcactttgact |
| STL1-E4 | CAGATCCGCCAGGCGTGTA GactCTCGAGcatttggcccatttggctcactttgact |
| STL1-E5 | CAGATCCGCCAGGCGTGTA |
| RTC3-803 | GactGTCGACtttattacttccatttac |
| RTC3-707 | gactGTCGACtgtatttgtcgaaaattt |
| RTC3-389 | gactGTCGACgaaagcgcaggttgaaac |
| RTC3-329 | gactGTCGACgcaattgtccctttctgac |
| RTC3-314 | gactGTCGACtgacaataagaCCCCTta |
| RTC3-197 | gactGTCGACTtatttagtcgaagggat |
| RTC3-157 | gactGTCGACGatatttaagtgatgagaa |
| RTC3-R | gactGGATCCggtcatgttgattttattttgtgtatg |
| HOT1-pt-F | gactGGATCCtcatgttttccattaatc |
| HOT1-R | gactGGATCCgttattgccagaatcattg |
| HOT1-F(Nco) | gactCCATGGctTCTGGAATGGGTATTGCG |
| HOT1-R2(N1) | gactGCGGCCGCacccttctcagaataag |
| Primers for mutagenesis | |
| STL1-STRE-F | tttagcttcaattttgtcTcGttcaacgctgcttggcc |
| STL1-STRE-R | ggccaagcagcgttgaaCgAgacaaaattgaagctaaa |
| STL1-delR2-F | gttgtcccactattccaccgcactttgactcaacttgcg |
| STL1-delR2-R | cgcaagttgagtcaaagtgcggtggaatagtgggacaac |
| STL1-delR3-F | gttgtcccactattccaccgcaacttgcgtcattttaac |
| STL1-delR3-R | gttaaaatgacgcaagttgcggtggaatagtgggacaac |
| STL1-delR4-F | gaaataagtgccgttgtcccactttgactcaacttgcg |
| STL1-delR4-R | cgcaagttgagtcaaagtgggacaacggcacttatttc |
| STL1-delR5-F | gaaataagtgccgttgtcccaacttgcgtcattttaac |
| STL1-delR5-R | gttaaaatgacgcaagttgggacaacggcacttatttc |
| STL1-delSKO-F | cccatttggctcactttgttttaactgatatgaaggg |
| STL1-delSKO-R | cccttcatatcagttaaaacaaagtgagccaaatggg |
| Primers for RT-PCR | |
| ACT1-RF | GTGTGGGGAAGCGGGTAAGC |
| ACT1-RR | GTGGCGGGTAAAGAAGAAAATGGA |
| STL1-RF | GTTGCGGTATTTCATCAC |
| STL1-RR | CATAGTTGAACTGTTTACC |
| CWP1-RF | CTCCACTGCTTTGTCTGTCG |
| CWP1-RR | CAAGTATTGTAAATCCGAGC |
| HSP12-RF | CTGACGCAGGTAGAAAAGG |
| HSP12-RR | GAACCTTACCAGCGACCTTG |
| KDX1-RF | CACCGAGAGGTGTATTTTCC |
| KDX1-RR | GAGTTTCCTCGTTCGATTC |
| PNS1-RF | CCGTTTTGGGCCTCACAC |
| PNS1-RR | CAGACCAGTACCTCAAAG |
| PRM10-RF | GCTCTATCCCTCGTTCTC |
| PRM10-RR | CAGCAACTAGGCTTCGAC |
| GRE2-RF | GTGTTCGATATGGCAAAAG |
| GRE2-RR | CTTCTTAGAACCACAGTAG |
| THI4-RF | GAAGACGAAGGTGACTATG |
| THI4-RR | GAACAGTTTAACATTTGG |
| YLR042C-RF | GGAGGTGTAGGTTCAGTC |
| YLR042C-RR | CAAATAAGCGATTGTTTC |
| FIT1-RF | GCCGCTTTAGGCGAAAGTATT |
| FIT1-RR | CTTCAGTTACTGCGGAGGTTACC |
| GPD1-RF | TCAATTTTTGCCCCGTATCTG |
| GPD1-RR | GATAGCTCTGACGTGTGAATCAACA |
| GPP2-RF | CGACGTCGACGGTACCATTA |
| GPP2-RR | CGAAATCCCTCCAGAATGCA |
| RTC3-RF | GGGCGCTGCCTCCAA |
| RTC3-RR | CTTCGATCTTCTTGCCCTTACC |
| NQM1-RF | CATTACTGTTTTCCTTTAC |
| NQM1-RR | CAGTATAGTCTTTGCCTG |
| ICL1-RF | GACACCGTTCCAAACAAAG |
| ICL1-RR | CATCTCATCGAGTTCTTC |
| YNR034W-A-RF | CCAATCACCGAAGTATTG |
| YNR034W-A-RR | GATTGGTGACTTTTCGATG |
| YGR066C-RF | GAAATGACAACATTGAAG |
| YGR066C-RR | CTCGTAAACATCACAGTC |