TABLE 1.
Oligonucleotides used for constructing DNA probes
| Probe | Primera | Nucleotide sequence of primers | Size (bp) | Position | Prototype H. influenzae strain |
|---|---|---|---|---|---|
| hif BC | hiB-F | 5′-CGCCGCCTGTTGCTCGAGTG-3′ | 1,191 | 1571-3020 | Eagan (GenBank accession no. U13254) |
| hifC-R | 5′-CACGCCACGCACCACGGGGG-3′ | ||||
| hia | hia-F | 5′-CGCGGCTTGGGCTGGGTCATTTCT-3′ | 766 | 2201-2967 | Strain 11 (GenBank accession no. U38617) |
| hia-R | 5′-TCAGCCGTACCGTCAGCATTCAGTTCA-3′ | ||||
| hmwC | hmwC-F | 5′-TTATGGGCAGGGAATCAACAACTTT-3′ | 703 | 553-1255 | Strain 12 (GenBank accession no. AF 180945) |
| hmwC-R | 5′-CACTGCCCACATAATCATCTTCTACGA-3′ | ||||
| hmw1A | hmw1-F1 | 5′-CCACCGGTGATGATACCAGAGGTG-3′ | 923 | 1959-2881 | Strain 12 (GenBank accession no. U08876) |
| hmw1-R1 | 5′-CGGCTTTCCTGGAGCCAAAGGTGA-3′ | ||||
| hmw2A | hmw2-F2 | 5′-GTCGCCCAGGGCACTGTAACCATT-3′ | 731 | 2122-2852 | Strain 12 (GenBank accession no. U08875) |
| hmw2-R2 | 5′-CCGCCCAGAATGGATATGTTGTAG-3′ | ||||
| pepN | pepN-F | 5′-GATGGTCGCCATTGGGTGG-3′ | 918 | 1219-2137 | Rd (GenBank accession no. NC_000907) |
| pepN-R | 5′-GATCTGCGGTTGGCGGTGTGG-3′ |
a
Primers were named for their respective genes. F and R correspond to forward and reverse direction, respectively.