TABLE 1.
QRDR mutation patterns for gonococcal samples as determined by direct sequencing and real-time PCR detection with the ABI 7900HT sequence detection system
| Sample type | QRDR mutation patterna
|
|||||
|---|---|---|---|---|---|---|
|
gyrA
|
parC
|
|||||
| aa 91 (Ser)b | aa 95 (Asp) | aa 86 (Asp) | aa 87 (Ser) | aa 88 (Ser) | aa 91 (Glu) | |
| A (WT, Cips) | WT | WT | WT | WT | WT | WT |
| B (mutant, Cipi) | WT | GAC→AAC (Asp→Asn) | WT | WT | WT | WT |
| C (mutant, Cipr) | TCC→TTC (Ser→Phe) | GAC→GGC (Asp→Gly) | GAC→AAC (Asp→Asn) | WT | WT | WT |
a
WT denotes the WT sequence at targeted loci.
b
The WT amino acid is listed in parentheses.