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Staining of E. bieneusi spores during various stages of the spore purification procedure. Stool preparation: calcofluor (a), Gram chromotrope (b), and IIF (c). Cesium chloride gradient centrifugation: calcofluor (d), Gram chromotrope (e), and IIF (f). Sorted material: calcofluor (g), Gram chromotrope (h), and IIF (i).
