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. 2015 Apr 13;125(5):1987–1997. doi: 10.1172/JCI73665

Figure 4. HIF2α inhibitor compound 76 suppresses the expression of HIF target genes in vhl–/– embryos.

Figure 4

(A) Morphologic differences between vhl–/– embryos and their siblings (WT or vhl+/– embryos) at 7 dpf. (B) Expression profile of zebrafish orthologs of human HIF1a (hif1aa and hif1ab) and human HIF2a (epas1a and epas1b) during development of vhl–/– embryos and their siblings. (C) Treatment protocol. vhl–/– embryos and their siblings were treated with compound 76 or DMSO vehicle control from 3 to 7 dpf, at the indicated concentrations. (D and E) Expression of the HIF target genes (D) phd3 and (E) epo, transferrin, vegfab, angiopoietin 1, and tgfa in 7-dpf vhl–/– embryos and their siblings treated with compound 76 at the indicated concentrations or vehicle-only control. Gene expression levels were obtained by qRT-PCR and normalized to the expression of 18S. All experiments were performed in biological triplicate. Data represent mean ± SEM. *P < 0.05, **P < 0.01, paired, 2-tailed t test.