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. 2015 Apr 20;125(5):2077–2089. doi: 10.1172/JCI77181

Figure 4. Progressive immunosuppression is associated with myeloma progression in vivo.

Figure 4

(A and B) Representative FACS plots (A) and graphs (B) showing the percentages of TCRβ+CD8+ cells and NK1.1+TCRβ– NK cells in the peripheral blood of Cd226+/+ Vk*MYC mice (n = 20) and nontransgenic control mice (n = 7) at 600 and 700 days. (C) Graphs showing the numbers of TCRβ+CD8+ cells and NK1.1+TCRβ– NK cells in the BM of naive mice or mice injected with Vk12653 MM cells for 5 weeks. Representative experiment out of 2 involving groups of n = 5–10 mice. (D) Graphs showing the correlation between the percentage of NK and CD8+ T cells and the number of BM PCs in the BM of mice injected with Vk12653 cells for 5 weeks (n = 61). Pearson r coefficient and associated P values are shown. (E) Graphs showing the mean fluorescence intensity (MFI) of CD226 on CD8+ T cells and NK cells as in A. (F) Representative histogram plots and graphs showing the MFI of CD226 staining on NK cells and CD8+ T cells as in C. Isotype control staining (gray line), and CD226 staining on naive mice (black line) and MM-bearing mice (dotted line) are shown. (G) Graph showing the correlation between the expression of CD226 on NK cells and CD8+ T cells and the number of BM PCs in the BM of mice injected with Vk12653 cells for 5 weeks (n = 20). Pearson r coefficient and associated P values are shown. Each symbol represents 1 individual mouse. *P < 0.05, **P < 0.01, ***P < 0.001; paired Student’s t test (B and E) and Mann-Whitney U test (C and F).