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. 2015 Apr 6;125(5):1927–1943. doi: 10.1172/JCI77767

Figure 4. Trailblazer cells express higher levels of genes required for collective invasion.

Figure 4

(A) Heat map showing the expression of the 26 genes that are specifically elevated in SUM159 and HCC1143 trailblazer cells. Expression is the mean of 2 biological replicates. (B) The relative invasion of SUM159 trailblazer cells ≥50 μm into the ECM. The number of invasive cells is normalized to the total cell number in the field of view for each condition. Relative invasion is the normalized invasive value of the test condition divided by the normalized invasion value of the control cells from the experimental replicate (median ± range, n = 3). The dashed red box indicates genes that were further investigated. Images are representative x-z views of SUM159 trailblazer invasion into ECM after transfection with the indicated siRNAs. For images of additional siRNA-transfected cells, see Supplemental Figure 5. Scale bar: 50 μm. (C) The relative invasion of SUM229 trailblazer cells ≥40 μm into the ECM normalized to the total cell number and compared with control cells (mean ± SD, n = 3). (D) Heat map showing that genes required for trailblazer cell invasion are highly expressed in 578T cells. Expression is the mean of 2 biological replicates. (E) The relative invasion of 578T cells ≥50 μm into the ECM normalized to the total cell number and compared with control cells (mean ± SD, n = 3).