TABLE 3.

Data for the ESBL-producing-isolates analyzed in detail

Isolate no.	Date of isolation (mo.yr)	Source	Warda	Mating	β-Lactamase pI by IEFb	ESBL identified by:		RAPD type	PFGE type
						PCR	Sequencing
60386	12.97	Nose swab	Internal medicine-diabetology (1)	Yes	9.0 + 6.0 + 5.4	TEM	TEM-47	J	f
4597	07.98	Urine	Internal medicine (2)	Yes	9.0 + 8.4 + 5.4	CTX-M	NDc	R	h
5970	09.98	Necrotic tissues	General surgery (2)	ND	9.0 + 8.4 + 5.4	CTX-M	CTX-M-3	X	ND
8096	12.98	Urine	Urology (2)	Yes	9.0 + 8.4 + 5.4	CTX-M	ND	X	ND
589	02.99	Urine	Internal medicine (2)	Yes	9.0 + 8.4 + 5.4	CTX-M	CTX-M-3	X	h
11689	05.99	Tracheal aspirate	Neurology (1)	Yes	9.0 + 8.4 + 5.4	CTX-M	CTX-M-3	J	f
14484	06.99	Throat swab	General surgery (1)	Yes	9.0 + 8.4 + 5.4	CTX-M	CTX-M-3	A	a
2362/b	06.99	Tracheal aspirate	ICU (1)	Yes	9.0 + 8.4 + 5.4	CTX-M	ND	X	h
18414	08.99	Sputum	Internal medicine-diabetology (1)	Yes	9.0 + 8.4 + 5.4	CTX-M	ND	A	a
23313	10.99	Blood	Internal medicine-diabetology (1)	Yes	9.0 + 8.4 + 5.4	CTX-M	CTX-M-3	X	h
4615	11.99	Blood	ICU (1)	No	9.0 + 8.4	CTX-M	CTX-M-3	X	h
7933	03.00	Drainage	General surgery (1)	Yes	9.0 + 8.4 + 5.4	CTX-M	ND	X	h
10827	05.00	Ear swab	Pediatric cardiology (1)	Yes	9.0 + 8.2 + 5.4	SHV	SHV-5	AR	ml
15219	07.00	Tracheal aspirate	Neurosurgery (1)	Yes	9.0 + 8.4 + 5.4	CTX-M	ND	J	f
15931	07.00	Urine	Pediatric nephrology (1)	Yes	9.0 + 8.4 + 5.4	CTX-M	CTX-M-3	BG	p
17349	07.00	Urine	Pediatric hematology (1)	No	9.0 + 6.0	TEM	TEM-47	AR	m
3628	08.00	Wound fistula	Internal medicine (2)	Yes	9.0 + 8.4 + 5.4	CTX-M	CTX-M-3	X	h
20152	08.00	Tracheal aspirate	Traumatic surgery (1)	Yes	9.0 + 8.4 + 5.4	CTX-M	ND	J	f
30594	12.00	Wound	General surgery (1)	Yes	9.0 + 8.4 + 5.4	CTX-M	CTX-M-3	A	r

^a(1) and (2), hospital 1 and hospital 2, respectively.

^bThe underlined values are pIs for the enzymes that were also observed in extracts of the transconjugants.

^cND, not done.