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. 2015 Apr 20;125(5):2136–2150. doi: 10.1172/JCI78558

Figure 4. Pendrin translocates to the apical membrane in SPAK KO mice.

Figure 4

(A) Immunolocalization of pendrin (red) in typical CNTs of WT and SPAK KO mice. Scale bars: 10 μm. (B) Quantitative image analysis of pendrin. Pendrin pixel intensity was measured over a 6-μm distance from the tubule lumen into the cytoplasm at 0.4 μm–increment resolution. The bracketed area delineates the apical membrane. (C) Summary data of apical membrane–delimited pendrin abundance. Apical pendrin increased by 55% in SPAK KO mice compared with that in WT. Data represent the mean ± SEM. n = 5 animals per genotype with 50 or more cells from each animal. *P > 0.05 by 2-tailed t test for WT versus KO. (D) Cl– absorption (JCl) was measured in isolated CCDs from WT and SPAK KO mice and perfused in vitro. Data represent the mean ± SEM. n = 4 animals per genotype. *P < 0.05 by 2-tailed t test for WT versus KO.