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. 2015 Apr 20;125(5):2136–2150. doi: 10.1172/JCI78558

Figure 7. ENaC expression and function are enhanced in SPAK KO mice.

Figure 7

(A) β-ENaC was identified in the SPAK KO profile as an upregulated gene. qPCR screening of all ENaC subunits verified the upregulation of the β subunit but also identified the γ subunit as upregulated in SPAK KO mice. Data represent the mean ± SEM. n = 6 animals. *P < 0.05 by 2-tailed t test for WT versus KO. The 24-hour diuretic (B) and natriuretic (C) responses of WT and SPAK KO mice to a single dose (1.0 μg/mg) of benzamil showed that basal urine volumes and UNaV were not different between the 2 animal groups. Additionally, both groups had demonstrated a significant increase in urine and sodium excretion when treated with benzamil. However, the change in urine and sodium excretion was significantly higher in SPAK KO mice than that in WT mice, suggesting increased ENaC-dependent sodium reabsorption in SPAK KO mice. Data represent the mean ± SEM. n = 5 animals. *P < 0.05 by 2-tailed t test, comparing the change in diuretic (B) and the change in natriuretic response (C) in WT versus KO mice.