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. 2015 Apr 6;125(5):1955–1967. doi: 10.1172/JCI78863

Figure 5. SEC63 and XBP1 regulate autoproteolysis of EMR2 and CL1 adhesion GPCRs.

Figure 5

(A) WT and DKO cells were transfected with WT EMR2 and the cleavage-deficient S518A mutant, both with COOH-terminal myc epitope tags. (B) WT and DKO cells were transfected with CL1 and the ΔGPS cleavage–deficient mutant expression vectors with HA-epitope tags in the COOH terminus. Cell lysates were analyzed by immunoblotting. *Cell lines contained the Pkd1F/H-BAC and HA immunoblotting detected the PC1-CTF expressed in WT cells.