Figure 8. Suppression of protein synthesis reduces basal IRE1α activity in SEC63-deficient cells.

(A) WT and (B) SEC63-KO cells were treated with DMSO, cycloheximide (50 μg/ml), tunicamycin (3 μg/ml), or both cycloheximide and tunicamycin for 4 hours. UPR activation was evaluated by immunoblotting.