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. 2015 Apr 20;125(5):2109–2122. doi: 10.1172/JCI79134

Figure 5. TERT affects MYC steady-state levels.

Figure 5

(A) Western blots showing levels of indicated proteins upon knockdown with shControl, shTERT1, shTERT2, shTERT3, and shTerc and upon reexpression of TERT WT and TERT DN in shTERT3 cells. (B) Western blots showing levels of indicated proteins upon TERT WT and TERT DN ectopic expression. TT, TERT+Terc. (C) Gene expression analysis of MYC in P493 cells infected with shControl, shTERT, and shTerc. #P > 0.05, compared with shControl, Student’s t test. (D) Western blot showing steady-state levels of MYC following TERT knockdown in EμMYC B cells using 2 independent shRNAs and in cells with reexpression of human TERT. (E) Coimmunoprecipitation between endogenous MYC and TERT in the cells infected with shControl, shTERT, or shTerc. (F and G) ChIP using antibodies against TERT and MYC, followed by qRT-PCR with primers specific for gene promoters containing MYC-binding sites in P493 siControl and siTERT cells. (H) Sequential ChIP was performed in P493 cells. ChIP was carried out first with antibody against MYC followed by antibody against either TERT or IgG as indicated. For the Western blots, representative images of n = 3 independent experiments are shown. *P < 0.05, compared with sh/siControl, Student’s t test.