Skip to main content
. 2015 Apr 20;125(5):2109–2122. doi: 10.1172/JCI79134

Figure 6. TERT regulates MYC half-life and ubiquitination.

Figure 6

(A and B) Western blots showing MYC levels upon TERT or Terc knockdown following cycloheximide (CHX) treatment for indicated time points in P493 cells and EμMYC B cells. UT, untreated. (C and D) Western blots in P493 and EμMYC B cells showing the levels of MYC, TERT, and actin upon TERT knockdown, with and without MG132 treatment (10 μM, 2 hours). (E) Western blots showing levels of indicated proteins upon vector, TERT WT, and TERT DN expression. (F) Coimmunoprecipitation between endogenous MYC and GSK3β in cells infected with shRNA against control (–) or TERT (+) and immunoblotting with indicated antibodies. (G) 293T cells were transfected with HA-MYC and His-ubiquitin, along with shControl or shTERT1. 48 hours after transfection cells were harvested, immunoprecipitated with Ni-NTA beads, and Western blotted with HA. (H) In vivo MYC ubiquitination assay in cells transfected with control or TERT shRNAs, along with HA-ubiquitin. Immunoprecipitation and immunoblotting from lysates or immunoprecipitated material were performed by using indicated antibodies. For the Western blots, representative images of n = 3 independent experiments are shown.