TABLE 2.

PCR primers

Primer	Sequence (5′-3′)	Specificity	Locationa (5′-3′)	Region of 16S rRNAb	Annealing temp (°C)	Amplicon sizec (bp)
UniF	GAGAGTTTGATCCTGGCTCAG	Most bacteria	7-27	5′-V1	60	391-406
LactoF	TGGAAACAGRTGCTAATACCG	All lactobacilli	157-167	V2.1-V2.2	62	231-233
LactoR	GTCCATTGTGGAAGATTCCC	All lactobacilli	379-360	V2.2-V3
LcaseF	GCACCGAGATTCAACATGG	L. casei/L. paracasei	86-104	V1	60	117
LrhamF	TGCTTGCATCTTGATTTAATTTTG	L. rhamnosus	81-104	V1	62	122
LcaseR	GGTTCTTGGATYTATGCGGTATTAG	L. casei group	196-169	V2.2
LfermR	GCACCTGATTGATTTTGGTCG	L. fermentum	86-94	V1	60	317
LdelbF	GGRTGATTTGTTGGACGCTAG	L. delbrueckii	92-102	V1	60	138
LdelbR	GCCGCCTTTCAAACTTGAATC	L. delbrueckii	219-199	V2.2
LgassR	CAGTTACTACCTCTATCTTTCTTCACTAC	L. gasseri	470-442	V3	60	322
LsaliF	CGAAACTTTCTTACACCGAATGC	L. salivarius	67-91	V1	60	332
LcrisF	AGCGAGCGGAACTAACAGATTTAC	L. crispatus	65-89	V1	60	154
LgallF	CGGTAATGACGCTGGGGAC	L. gallinarum	78-97	V1	64	128
LultF	GAGCGGAACCAGCAGATCTG	L. ultunensis	68-87	V1	60	151
LcrisR	AGCTGATCATGCGATCTGCTT	L. acidophilus groups A1-A4d	205-185	V2.2
T7 primer	TAATACGACTCACTATAGGG	T7 promoter

^aLocations relative to positions in the E. coli 16S rRNA gene.

^bVariable regions according to Neefs et al. (28).

^cTheoretical amplicon sizes based on the reference sequence for that species; for L. gasseri, L. salivarius, and L. fermentum, either LactoF or LactoR was the complementary primer.

^dL. acidophilus groups according to Johnson et al. (15).