TABLE 3.

Lactobacilli detected in carious dentine by real-time PCR

Species	No. of positive samples (%)	DNA (pg [mg(wet wt) of dentine]−1)			Cell equivalentsa (mg [wet wt] of dentine)−1
		Range	Median	Mean ± SEM	Range	Median	Mean ± SEM
Total lactobacilli	65 (100)	25-3.6 × 105	1.5 × 104	4.7 × 104 ± 7.0 × 104	1.0 × 104-1.4 × 108	5.9 × 106	1.9 × 107 ± 2.8 × 107
L. rhamnosus	35 (54)	2.0 × 102-3.4 × 104	4.1 × 103	7.6 × 103 ± 8.8 × 103	7.6 × 104-1.3 × 107	1.5 × 106	2.9 × 106 ± 3.4 × 106
L. casei/L. paracasei	26 (40)	4.0-2.2 × 104	6.4 × 102	2.7 × 103 ± 5.1 × 103	1.4 × 103-7.6 × 106	2.2 × 105	9.6 × 105 ± 17.6 × 105
L. fermentum	14 (22)	1.5 × 102-4.7 × 104	9.3 × 102	4.7 × 102 ± 12.4 × 103	6.3 × 104-2.0 × 107	3.9 × 105	1.9 × 106 ± 5.1 × 106
L. delbrueckii	4 (6)	1.0-8.8 × 104	5.9 × 10	2.1 × 104 ± 3.7 × 104	3.8 × 102-3.5 × 107	2.2 × 104	8.4 × 106 ± 14.8 × 106
L. gasseri	35 (54)	6.1 × 10-1.6 × 105	7.4 × 103	1.7 × 104 ± 3.0 × 104	3.1 × 104-8.0 × 107	3.8 × 106	8.7 × 106 ± 15.2 × 106
L. salivarius	39 (60)	1.3 × 10-5.3 × 104	2.1 × 103	9.2 × 103 ± 12.5 × 103	5.5 × 103-2.2 × 107	8.7 × 105	3.8 × 106 ± 5.2 × 106
L. crispatus	29 (45)	1.4 × 10-3.9 × 104	2.1 × 103	5.9 × 103 ± 9.0 × 103	5.9 × 103-1.6 × 107	8.5 × 105	2.4 × 106 ± 3.7 × 106
L. gallinarum	6 (9)	1.4 × 102-2.6 × 104	3.3 × 103	8.4 × 103 ± 10.8 × 103	5.7 × 104-1.1 × 107	1.4 × 106	3.5 × 106 ± 4.5 × 106
L. ultunensis/Lactobacillus sp. oral clone	33 (51)	5.3 × 10-1.8 × 105	3.2 × 103	2.0 × 104 ± 3.5 × 104	2.2 × 104-7.4 × 107	1.3 × 106	8.1 × 106 ± 14.4 × 106

^aThe genome sizes assumed for calculating cell numbers were as follows: L. casei, 2.6 Mb; L. rhamnosus, 2.4 Mb; L. fermentum, L. salivarius, L. crispatus, L. gallinarum and L. ultunensis, 2.2 Mb; L. gasseri, 1.8 Mb; and L. delbrueckii, 2.3 Mb.