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. 2015 Apr 6;125(5):1979–1986. doi: 10.1172/JCI80743

Figure 1. The mHTT SMC immunoassay is specific and sensitive.

Figure 1

(A) Schematic of mHTT (not to scale) indicating the epitopes bound by the 2B7 and MW1 antibodies used for the assay. 2B7 binds residues 1–17 of the N terminus (11), while MW1 conveys specificity for the mutant protein by binding preferentially to the expanded polyglutamine tract (13). (B) Detection of purified recombinant HTT proteins with the SMC immunoassay shows specificity for mutant (mHTT) over WT human HTT protein. (C) Determination of the assay’s LLoQ and ULoQ shows a low femtomolar detection threshold and broad dynamic range.