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Analysis of cDNA products corresponding to the native sites of 2’-O-methylation in 28S rRNA. A – 2’-O-methylated nucleotides in 28S rRNA and lengths of corresponding termination products of RT with primer FAM-4491. B–D – analysis of FAM-labeled products of RT termination separated on an automated DNA analyzer. RT of 28S rRNA isolated from MCF-7 cells was conducted with the following concentrations of dNTP in the reaction mixture: B – 0.1 mM, C – 1.0 mM, D – 2.0 mM
