Table.
Yields of the RT termination product corresponding to Cm4506 of 28S rRNA in MCF-7 cells transfected with box C/D RNA analogues
|
Name of box C/D RNA analogue |
Alteration in box C/D RNA structure compared to RNA5 |
Yield of the RT termination product corresponding to Cm4506 28S rRNA** |
Relative yield of the RT termination product ***, % |
|---|---|---|---|
| RNA5 | - | 1100 ± 154 | 100 |
| RNA3 |
3-nucleotide shift to the 5’-end of rRNA in the complementary region |
6500 ± 780 | 590 |
| RNA5mC |
box C (AUGAUGU) → (ACAGCAC); box C’ (GUGAUGA) → (GCAGCAG) |
170 ± 25 | 15 |
| RNA5D/N | box D (CUGA) → (AGUC) | 570 ± 86 | 50 |
| RNA5mD | Boxes D and D’ (CUGA) → (AAAA) | 150 ± 25 | 13 |
| Control* | - | 150 ± 25 | 13 |
* Control cells were incubated with lipofectamine alone.
** Values of peak areas normalized to the total area of the peaks of RT termination products (±SD).
*** Relative yields of the RT termination product corresponding to Cm4506 28S rRNA compared to the yield of the same product of the RT of 28S rRNA isolated from MCF-7 cells transfected with RNA5.