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. 2015 Apr 24;290(24):14826–14840. doi: 10.1074/jbc.M114.630640

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — DNA damage-induced HUS1 localization is defective in HUS1 clamp-forming and DNA-interacting mutants but not in HUS1 pocket mutants. A and B, MMC-treated Hus1-null MEFs stably expressing the indicated constructs were stained with DAPI (blue) and α-FLAG (green) and α-RAD9A (red) antibodies. Scale bar, 10 μm. C and D, quantifications of colocalized FLAG and RAD9A foci are presented in quartile box and dot plots. NS, not significant. D, immunoblotting using antibodies specific for FLAG or β-actin was performed to compare the stability of 3XFLAG-tagged WT and mutant mHUS1 proteins (C1, C4, and C5) in HEK293T cells. E, cells were UV-treated; fractionated into cytoplasmic (cyt), nuclear (nuc), and chromatin (chr) fractions; and immunoblotted. GAPDH and histone 3 served as fractionation controls. Arrows, HUS1 band; asterisks, nonspecific band.