FIGURE 9.

MZsphk2 embryos exhibit the tail blister phenotype. A–G, lateral bright field images of the medial fin at 2 dpf embryos. MZsphk2, zygotic spns2 (Zspns2), and zygotic s1pr2 (Zs1pr2) embryos show the tail blister phenotype, whereas wt, MZsphk1, zygotic sphk2 (Zsphk2), and Msphk2 embryos show normal fins. H, embryos exhibiting the tail blister (black) or normal fin (gray) phenotypes were quantified. The total number of embryos is shown on the top of each column. K1, sphk1; K2, sphk2; S2, spns2; R2, s1pr2. Mating was performed as indicated below the column (+, wild-type allele; −, mutant allele). Scale bar, 200 μm.